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aedes albopictus c6 36 mosquito cell line  (ATCC)


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    ATCC aedes albopictus c6 36 mosquito cell line
    Aedes Albopictus C6 36 Mosquito Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1385 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c6+36+mosquito+cell+line/Aedes+albopictus+clone+C6%2F36/pm42262762-45-29-35
    Average 97 stars, based on 1385 article reviews
    aedes albopictus c6 36 mosquito cell line - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Virus:

    Article Title: Zika virus-specific IgM elicited during pregnancy exhibits ultrapotent neutralization
    Article Snippet: .. Inactivation of virus was verified by serial passaging of virus on C6/36 mosquito cell line (ATCC), without detection of infection on this susceptible cell line. .. Thawed PBMCs were stained with Aqua Vital Dye (Invitrogen), IgD-PE (clone IA6-2; BD Biosciences), CD10 PE-CF594 (clone HI10a; BD Biosciences; CD3 PE-Cy5 (clone HIT3a; BD Biosciences), CD14 BV605 (clone M5E2; BD Biolegend), CD16 BV570 (clone 3G8; Biolegend), CD27 PE-Cy7 (clone O323; Thermo Fisher Scientific), CD38 APC-AF700 (clone LS198-4-3; Beckman Coulter), CD19 APC-Cy7 (clone SJ25C1; BD Biosciences) and 1 x 10 6 PFU of freshly thawed UV-inactivated ZIKV labelled with AF488.

    Article Title: Attenuated variant of the rift valley fever virus, composition comprising same, and uses thereof
    Article Snippet: Cells, Viruses and Infections Vero cells (ATCC No. Catalogue CCL-81) were grown in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 5% to 10% fetal calf serum (FCS) and L-glutamine (2 mM), penicillin (100 U/ml) and streptomycin (100 g/ml) in a humid atmosphere of 5% CO2 at 37° C. Insect cells of Aedes albopictus C6/36 (ATCC No. Catalogue CRL1660) were grown in Eagle minimal essential medium (EMEM) supplemented with 10% fetal calf serum (FCS), L-glutamine (2 mM), gentamicin (50 μg/ml) and vitamin MEM solution (Sigma) in a humid atmosphere of 5% CO2 at 28° C. The starting parental virus originated in a sheep experimentally infected with wild strain 56/74 isolate of the VRVR 56/74 virus (parental virus) (Borrego et al., 2019); (Busquets et al., 2010). .. The virus was re-isolated from infected sheep plasma and cultured in a C6/36 mosquito cell line (ATCC CRL-1660). .. Assays to quantify plate-forming units (pfu) were performed in semi-solid medium including 1% carboxymethylcellulose (CMC; Sigma). pfu units are used in virology to describe the number of virus particles capable of forming plates per unit volume.

    Article Title: The route of infection shapes Rift Valley fever virus pathogenesis, humoral immune response, and horizontal transmission in sheep
    Article Snippet: .. The virus used in our experiment was isolated in the C6/36 mosquito cell line (Aedes albopictus clone, ATCC CRL-1660TM) from the plasma of a sheep infected with RVFV (strain 56/74) [ ]. .. RVFV-56/74 stock was produced in C6/36 cells grown in Dulbecco’s modified Eagle medium (DMEM, Bio-West, Nuaillé, France) supplemented with heat inactivated 10% foetal bovine serum (FBS, GibcoTM-Thermo Fisher Scientific.

    Article Title: A Zika virus-specific IgM elicited in pregnancy exhibits ultrapotent neutralization.
    Article Snippet: .. Inactivation of virus was verified by serial passaging of virus on C6/36 mosquito cell line (ATCC), without detection of infection on this susceptible cell line. .. Staining and sorting ZIKV-reactive B cells Thawed PBMCs were stained with Aqua Vital Dye (Invitrogen), IgD-PE (clone IA6-2; BD Biosciences), CD10 PE-CF594 (clone HI10a; BD Biosciences); CD3 PE-Cy5 (clone HIT3a; BD Biosciences), CD14 BV605 (clone M5E2; BD Biolegend), CD16 BV570 (clone 3G8; Biolegend), CD27 PE-Cy7 (clone O323; Thermo Fisher Scientific), CD38 APC-AF700 (clone LS198-4-3; Beckman Coulter), CD19 APC-Cy7 (clone SJ25C1; BD Biosciences) and 13 106 PFU of freshly thawed UV-inactivated ZIKV labeled with AF488.

    Passaging:

    Article Title: Zika virus-specific IgM elicited during pregnancy exhibits ultrapotent neutralization
    Article Snippet: .. Inactivation of virus was verified by serial passaging of virus on C6/36 mosquito cell line (ATCC), without detection of infection on this susceptible cell line. .. Thawed PBMCs were stained with Aqua Vital Dye (Invitrogen), IgD-PE (clone IA6-2; BD Biosciences), CD10 PE-CF594 (clone HI10a; BD Biosciences; CD3 PE-Cy5 (clone HIT3a; BD Biosciences), CD14 BV605 (clone M5E2; BD Biolegend), CD16 BV570 (clone 3G8; Biolegend), CD27 PE-Cy7 (clone O323; Thermo Fisher Scientific), CD38 APC-AF700 (clone LS198-4-3; Beckman Coulter), CD19 APC-Cy7 (clone SJ25C1; BD Biosciences) and 1 x 10 6 PFU of freshly thawed UV-inactivated ZIKV labelled with AF488.

    Article Title: A Zika virus-specific IgM elicited in pregnancy exhibits ultrapotent neutralization.
    Article Snippet: .. Inactivation of virus was verified by serial passaging of virus on C6/36 mosquito cell line (ATCC), without detection of infection on this susceptible cell line. .. Staining and sorting ZIKV-reactive B cells Thawed PBMCs were stained with Aqua Vital Dye (Invitrogen), IgD-PE (clone IA6-2; BD Biosciences), CD10 PE-CF594 (clone HI10a; BD Biosciences); CD3 PE-Cy5 (clone HIT3a; BD Biosciences), CD14 BV605 (clone M5E2; BD Biolegend), CD16 BV570 (clone 3G8; Biolegend), CD27 PE-Cy7 (clone O323; Thermo Fisher Scientific), CD38 APC-AF700 (clone LS198-4-3; Beckman Coulter), CD19 APC-Cy7 (clone SJ25C1; BD Biosciences) and 13 106 PFU of freshly thawed UV-inactivated ZIKV labeled with AF488.

    Infection:

    Article Title: Zika virus-specific IgM elicited during pregnancy exhibits ultrapotent neutralization
    Article Snippet: .. Inactivation of virus was verified by serial passaging of virus on C6/36 mosquito cell line (ATCC), without detection of infection on this susceptible cell line. .. Thawed PBMCs were stained with Aqua Vital Dye (Invitrogen), IgD-PE (clone IA6-2; BD Biosciences), CD10 PE-CF594 (clone HI10a; BD Biosciences; CD3 PE-Cy5 (clone HIT3a; BD Biosciences), CD14 BV605 (clone M5E2; BD Biolegend), CD16 BV570 (clone 3G8; Biolegend), CD27 PE-Cy7 (clone O323; Thermo Fisher Scientific), CD38 APC-AF700 (clone LS198-4-3; Beckman Coulter), CD19 APC-Cy7 (clone SJ25C1; BD Biosciences) and 1 x 10 6 PFU of freshly thawed UV-inactivated ZIKV labelled with AF488.

    Article Title: Attenuated variant of the rift valley fever virus, composition comprising same, and uses thereof
    Article Snippet: Cells, Viruses and Infections Vero cells (ATCC No. Catalogue CCL-81) were grown in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 5% to 10% fetal calf serum (FCS) and L-glutamine (2 mM), penicillin (100 U/ml) and streptomycin (100 g/ml) in a humid atmosphere of 5% CO2 at 37° C. Insect cells of Aedes albopictus C6/36 (ATCC No. Catalogue CRL1660) were grown in Eagle minimal essential medium (EMEM) supplemented with 10% fetal calf serum (FCS), L-glutamine (2 mM), gentamicin (50 μg/ml) and vitamin MEM solution (Sigma) in a humid atmosphere of 5% CO2 at 28° C. The starting parental virus originated in a sheep experimentally infected with wild strain 56/74 isolate of the VRVR 56/74 virus (parental virus) (Borrego et al., 2019); (Busquets et al., 2010). .. The virus was re-isolated from infected sheep plasma and cultured in a C6/36 mosquito cell line (ATCC CRL-1660). .. Assays to quantify plate-forming units (pfu) were performed in semi-solid medium including 1% carboxymethylcellulose (CMC; Sigma). pfu units are used in virology to describe the number of virus particles capable of forming plates per unit volume.

    Article Title: The route of infection shapes Rift Valley fever virus pathogenesis, humoral immune response, and horizontal transmission in sheep
    Article Snippet: .. The virus used in our experiment was isolated in the C6/36 mosquito cell line (Aedes albopictus clone, ATCC CRL-1660TM) from the plasma of a sheep infected with RVFV (strain 56/74) [ ]. .. RVFV-56/74 stock was produced in C6/36 cells grown in Dulbecco’s modified Eagle medium (DMEM, Bio-West, Nuaillé, France) supplemented with heat inactivated 10% foetal bovine serum (FBS, GibcoTM-Thermo Fisher Scientific.

    Article Title: A Zika virus-specific IgM elicited in pregnancy exhibits ultrapotent neutralization.
    Article Snippet: .. Inactivation of virus was verified by serial passaging of virus on C6/36 mosquito cell line (ATCC), without detection of infection on this susceptible cell line. .. Staining and sorting ZIKV-reactive B cells Thawed PBMCs were stained with Aqua Vital Dye (Invitrogen), IgD-PE (clone IA6-2; BD Biosciences), CD10 PE-CF594 (clone HI10a; BD Biosciences); CD3 PE-Cy5 (clone HIT3a; BD Biosciences), CD14 BV605 (clone M5E2; BD Biolegend), CD16 BV570 (clone 3G8; Biolegend), CD27 PE-Cy7 (clone O323; Thermo Fisher Scientific), CD38 APC-AF700 (clone LS198-4-3; Beckman Coulter), CD19 APC-Cy7 (clone SJ25C1; BD Biosciences) and 13 106 PFU of freshly thawed UV-inactivated ZIKV labeled with AF488.

    Clinical Proteomics:

    Article Title: Attenuated variant of the rift valley fever virus, composition comprising same, and uses thereof
    Article Snippet: Cells, Viruses and Infections Vero cells (ATCC No. Catalogue CCL-81) were grown in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 5% to 10% fetal calf serum (FCS) and L-glutamine (2 mM), penicillin (100 U/ml) and streptomycin (100 g/ml) in a humid atmosphere of 5% CO2 at 37° C. Insect cells of Aedes albopictus C6/36 (ATCC No. Catalogue CRL1660) were grown in Eagle minimal essential medium (EMEM) supplemented with 10% fetal calf serum (FCS), L-glutamine (2 mM), gentamicin (50 μg/ml) and vitamin MEM solution (Sigma) in a humid atmosphere of 5% CO2 at 28° C. The starting parental virus originated in a sheep experimentally infected with wild strain 56/74 isolate of the VRVR 56/74 virus (parental virus) (Borrego et al., 2019); (Busquets et al., 2010). .. The virus was re-isolated from infected sheep plasma and cultured in a C6/36 mosquito cell line (ATCC CRL-1660). .. Assays to quantify plate-forming units (pfu) were performed in semi-solid medium including 1% carboxymethylcellulose (CMC; Sigma). pfu units are used in virology to describe the number of virus particles capable of forming plates per unit volume.

    Article Title: The route of infection shapes Rift Valley fever virus pathogenesis, humoral immune response, and horizontal transmission in sheep
    Article Snippet: .. The virus used in our experiment was isolated in the C6/36 mosquito cell line (Aedes albopictus clone, ATCC CRL-1660TM) from the plasma of a sheep infected with RVFV (strain 56/74) [ ]. .. RVFV-56/74 stock was produced in C6/36 cells grown in Dulbecco’s modified Eagle medium (DMEM, Bio-West, Nuaillé, France) supplemented with heat inactivated 10% foetal bovine serum (FBS, GibcoTM-Thermo Fisher Scientific.

    Cell Culture:

    Article Title: Attenuated variant of the rift valley fever virus, composition comprising same, and uses thereof
    Article Snippet: Cells, Viruses and Infections Vero cells (ATCC No. Catalogue CCL-81) were grown in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 5% to 10% fetal calf serum (FCS) and L-glutamine (2 mM), penicillin (100 U/ml) and streptomycin (100 g/ml) in a humid atmosphere of 5% CO2 at 37° C. Insect cells of Aedes albopictus C6/36 (ATCC No. Catalogue CRL1660) were grown in Eagle minimal essential medium (EMEM) supplemented with 10% fetal calf serum (FCS), L-glutamine (2 mM), gentamicin (50 μg/ml) and vitamin MEM solution (Sigma) in a humid atmosphere of 5% CO2 at 28° C. The starting parental virus originated in a sheep experimentally infected with wild strain 56/74 isolate of the VRVR 56/74 virus (parental virus) (Borrego et al., 2019); (Busquets et al., 2010). .. The virus was re-isolated from infected sheep plasma and cultured in a C6/36 mosquito cell line (ATCC CRL-1660). .. Assays to quantify plate-forming units (pfu) were performed in semi-solid medium including 1% carboxymethylcellulose (CMC; Sigma). pfu units are used in virology to describe the number of virus particles capable of forming plates per unit volume.

    Article Title: Cellular Organelles Reorganization During Zika Virus Infection of Human Cells
    Article Snippet: .. The C6/36 mosquito cell line (from Aedes albopictus , ATCC CRL-1660), adapted to grow at 33°C, was cultured in L-15 medium (Leibovitz; GIBCO) supplemented with 0.3% tryptose phosphate broth, 0.02% glutamine, 1% MEM non-essential amino acids solution and 10% FBS. ..

    Isolation:

    Article Title: The route of infection shapes Rift Valley fever virus pathogenesis, humoral immune response, and horizontal transmission in sheep
    Article Snippet: .. The virus used in our experiment was isolated in the C6/36 mosquito cell line (Aedes albopictus clone, ATCC CRL-1660TM) from the plasma of a sheep infected with RVFV (strain 56/74) [ ]. .. RVFV-56/74 stock was produced in C6/36 cells grown in Dulbecco’s modified Eagle medium (DMEM, Bio-West, Nuaillé, France) supplemented with heat inactivated 10% foetal bovine serum (FBS, GibcoTM-Thermo Fisher Scientific.



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